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Authors
Affiliations
1 Dept. of Pharmaceutical Chemistry, JKKMMRF College of Pharmacy, Komara Palayam-638183, Tamil Nadu, IN
2 Dept. of Pharmaceutical Chemistry, Maharaji College of Pharmacy, Chennai-600090, Tamil Nadu, IN
Source
Asian Journal of Research in Chemistry, Vol 2, No 4 (2009), Pagination: 469-471
Abstract
A simple, precise, accurate and rapid HPLC method has been developed, and validated for the determination of ofloxacin and satranidazole simultaneously, in combined dosage form. Acetonitrile and ammonium di-hydrogen orthophosphate buffer with ph 7.0 (50%:50%)is used as the mobile phase, 300 is the detection wavelength for this study. The applicability of the method for simultaneous determinations of oflaxacin and satranidazole was verified by the determination of these compounds in marketed tablets .Results of the analysis were validated statistically, and by recovery studies (99.43-101.28%). The recovery and RSD values with in limits given in ICH guidelines method developments indicates that the suitability of proposed for the routine determination of the these compounds in tablets .the validation parameters; linearity (r=9999) sensitivity (LOD (1.0-1.5×10-4), LOQ (3.0-4.5×10-4) accuracy (99.43-101.28%) and reproducibility were found to satisfactory. The proposed method can be successfully used to determine the drug contents of marketed formulation.
Keywords
RP-HPLC, Oflaxacin, Satranidazole, Validation.
